In vitro selection and evolution of functional proteins by using ribosome display
نویسندگان
چکیده
منابع مشابه
In-vitro protein evolution by ribosome display and mRNA display.
In-vitro display technologies combine two important advantages for identifying and optimizing ligands by evolutionary strategies. First, by obviating the need to transform cells in order to generate and select libraries, they allow a much higher library diversity. Second, by including PCR as an integral step in the procedure, they make PCR-based mutagenesis strategies convenient. The resulting ...
متن کامل27 In Vitro Selection and Evolution of Protein - . . Ligand Interactions by Ribosome Display
Ribosome display is a method for selecting and further evolving functional proteins for their properties of interaction and biophysical parameters. It is performed completely in vitro and does not involve living cells at any step (Hanes and Pliickthun 1997). The method has two main advantages over most other selection methods. First, the screening of very large libraries with up to 1014 members...
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Objective(s): Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for the 6-kDa early secretory antigenic target (ESAT-6) antigen of Mycobacterium tuberculosis from a mouse scFv library. Materials and Methods: The g...
متن کاملRibosome display: an in vitro method for selection and evolution of antibodies from libraries.
Combinatorial approaches in biology require appropriate screening methods for very large libraries. The library size, however, is almost always limited by the initial transformation steps following its assembly and ligation, as other all screening methods use cells or phages and viruses derived from them. Ribosome display is the first method for screening and selection of functional proteins pe...
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Although yeast two-hybrid assay and biochemical methods combined with mass spectrometry have been successfully employed for the analyses of protein-protein interactions in the field of proteomics, these methods encounter various difficulties arising from the usage of living cells, including inability to analyze toxic proteins and restriction of testable interaction conditions. Totally in vitro ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1997
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.94.10.4937